Premature translation of protamine 1 mRNA causes precocious nuclear condensation and arrests spermatid differentiation in mice.
نویسندگان
چکیده
منابع مشابه
Spermatid-specific expression of protamine 1 in transgenic mice.
Protamines are abundant basic proteins involved in the condensation of sperm chromatin. In the mouse, protamine genes are transcribed postmeiotically in round spermatids. We have cloned and sequenced the mouse protamine 1 gene. Ten lines of transgenic mice harboring marked protamine 1 sequences were generated by microinjection of fertilized eggs. Transcription of the transgene is restricted to ...
متن کاملEffect of Protamine Deficiency on Fertilization Outcome and Incidence of Sperm Premature Chromosomal Condensation Post ICSI
Purpose: After aneuploidy,sperm premature chromatin condensation (PCC) is the next most important cause of fertilization failure. Therefore the aim of this study is to evaluate the effect of sperm protamine deficiency on sperm PCC formation post ICSI. Materials and Methods: Cytogenetic analysis was carried out on unfertilized oocytes post ICSI and incidence of sperm PCC was evaluated. The resu...
متن کاملThe Effects of Protamine Deficiency in Sperm Humanon Premature Chronosomal Condensation
Purpose: After aneuploidy, failure of oocyte activation, resulting in premature chromosomal condensation (PCC), is considered as the second cause of failed fertilization post ICSI. Oocyte quality has been shown to affect PCC formation, and since this study sought to evaluate the effect of sperm chromatin anomalies on the PCC, mice metaphase II oocytes were used. Materials and Methods: Metaphas...
متن کاملComparison of Protamine 1 to Protamine 2 mRNA Ratio and YBX2 gene mRNA Content in Testicular Tissue of Fertile and Azoospermic Men
Background Although aberrant protamine (PRM) ratios have been observed in infertile men, the mechanisms that implicit the uncoupling of PRM1 and PRM2 expression remain unclear. To uncover these mechanisms, in this observational study we have compared the PRM1/PRM2 mRNA ratio and mRNA contents of two regulatory factors of these genes. MaterialsAndMethods In this experimental study, sampling was ...
متن کاملInactivation of a testis-specific Lis1 transcript in mice prevents spermatid differentiation and causes male infertility.
Lis1 protein is the non-catalytic component of platelet-activating factor acetylhydrolase 1b (PAF-AH 1B) and associated with microtubular structures. Hemizygous mutations of the LIS1 gene cause type I lissencephaly, a brain abnormality with developmental defects of neuronal migration. Lis1 is also expressed in testis, but its function there has not been determined. We have generated a mouse mut...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1995
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.92.26.12451